Evaluation of immunoassay methods for prolactin in body fluids. AMRL-TR-66-113.

A practical immunologic assay to detect the hormone, prolactin, in body fluids was evaluated. Prolactin content was assayed using positive antiprolactin sera prepared in rabbits and test samples of serum, urine, and urinary extract from a borane exposed female monkey in micro-Ouchterlony gel diffusion and agglutination inhibition tests. The gel diffusion test gave qualitative results while the agglutination inhibition test allowed quantitation of prolactin content. Results of the later test indicated a possible difference in preand postborane exposure prolactin content of monkey urine. Further work in this area will need to be done to clarify this preliminary evidence and to expand on its applicability to human exposure to boranes.